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Merck
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主要文件

395B

Sigma-Aldrich

周期性 酸-希夫(PAS)染色系统

Periodic Acid-Schiff (PAS) Staining System
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About This Item

UNSPSC代码:
12352106
NACRES:
NA.47
价格与库存信息目前不能提供

保质期

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

应用

hematology
histology

运输

wet ice

储存温度

2-8°C

应用

在包装说明书内含有标准操作步骤和微波操作步骤。
采用过碘酸处理时,乙二醇被氧化成醛。与Schiff′s Reagent(由碱性副品红和偏亚硫酸钠组成的混合物)反应后,碱性副品红化合物释放,将含有乙二醇的胞内组分染为红色。可通过PAS操作步骤染色胞内成分包括糖原、真菌细胞壁、基膜、某些上皮硫粘蛋白和唾液黏蛋白、中性粘液质、甲状腺的胶质材料以及脑垂体的中间部。

仅试剂盒组分

产品编号
说明

  • Hematoxylin Solution, Gill No. 3 (kit only) 100 mL

  • Periodic Acid Solution (395-1) 2 x 100

  • Schiff's Reagent (kit only) 4 x 50

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT RE 2 Oral

靶器官

Kidney

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ming Jiang et al.
Nature, 550(7677), 529-533 (2017-10-12)
In several organ systems, the transitional zone between different types of epithelium is a hotspot for pre-neoplastic metaplasia and malignancy, but the cells of origin for these metaplastic epithelia and subsequent malignancies remain unknown. In the case of Barrett's oesophagus
Luke Whitesell et al.
Nature communications, 10(1), 402-402 (2019-01-27)
New strategies are needed to counter the escalating threat posed by drug-resistant fungi. The molecular chaperone Hsp90 affords a promising target because it supports survival, virulence and drug-resistance across diverse pathogens. Inhibitors of human Hsp90 under development as anticancer therapeutics
Anja Beckers et al.
Development (Cambridge, England), 147(21) (2020-05-08)
Cilia are complex cellular protrusions consisting of hundreds of proteins. Defects in ciliary structure and function, many of which have not been characterised molecularly, cause ciliopathies: a heterogeneous group of human syndromes. Here, we report on the FOXJ1 target gene
Satish Patel et al.
PloS one, 6(1), e15845-e15845 (2011-01-22)
Over-activity and elevated expression of glycogen synthase kinase-3 (GSK-3) has been implicated in the etiology of insulin resistance and Type 2 diabetes. Administration of specific GSK-3 inhibitors to diabetic or obese rodent models improves glycaemic control and insulin sensitivity. However
Tavia Caplan et al.
Cell chemical biology, 27(3), 269-282 (2020-01-12)
New strategies are urgently needed to counter the threat to human health posed by drug-resistant fungi. To explore an as-yet unexploited target space for antifungals, we screened a library of protein kinase inhibitors for the ability to reverse resistance of

商品

Fungal stains remain an important tool in the histology laboratory's diagnostic arsenal for identifying infectious microorganisms.

Fungal stains remain an important tool in the histology laboratory's diagnostic arsenal for identifying infectious microorganisms.

Fungal stains remain an important tool in the histology laboratory's diagnostic arsenal for identifying infectious microorganisms.

Fungal stains remain an important tool in the histology laboratory's diagnostic arsenal for identifying infectious microorganisms.

Questions

1–2 of 2 Questions  
  1. How many slides can be stained using the PAS kit (395B-1kt)?

    1 answer
    1. The 395B kit is a kit formulated for multiple uses. Typically, the reagent is only replaced after the staining performance drops off. For kit 395B, calculating an exact range of slides is not possible. The kit is provided with 4 each of the 50 ml bottles of Schiffs reagent and 2 each for the 100 ml bottles for Periodic Acid. Each 50 ml aliquot is probably sufficient for 5-10 uses. The exact number will depend on several factors. If the slides are all to be stained at one time, a larger number of slides could be stained. If the slides are stained 2 or 3 at a time over a large number of weeks, fewer slides could be stained. Again, the solutions are to be replaced as needed and not necessarily after a set number of slides have been stained.

      Helpful?

  2. What product do you recommend for Periodic acid, Sodium acetate, and Ammonium hydroxide for PAS staining?

    1 answer
    1. For PAS staining, you can either obtain a kit (395B) or the individual reagents. The kit includes 1% Periodic Acid, Schiff's Reagent, and Gill's 3 Hematoxylin. Below are the various catalog numbers:

      •395B for the PAS kit, with Instructions for Use on the sigmaaldrich.com website.
      •3951-100ml or 395132-1L for the 1% Periodic Acid.
      •3952016-500ml for the Schiff's Reagent. Note that there are other Schiff's reagents for sale, but not all are intended for staining tissue.
      •GHS316-500ml for the Gill's 3 Hematoxylin, available separately from the kit.

      Helpful?

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