We have used this great product for years, even prior to the acquisition by Sigma. Would really appreciate the operations improving to have the 10L bag size available, even prior to the pandemic this has been very poorly handled. Lack of bags to fill, failing QC...so we adjust by buying the smaller size at much higher cost and risk failed runs by having to manipulate filling our own bio-bags with numerous 1L size bottles.
推荐产品
描述
for research or for further manufacturing use
质量水平
无菌性
sterile-filtered
产品线
EX-CELL®
表单
liquid
技术
cell culture | insect: suitable
组分
HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes
运输
ambient
储存温度
2-8°C
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应用
EX-CELL 420 是一种完全培养基， 是为了实现Sf9 和 Sf21昆虫细胞系在无血清条件下生长而开发和优化。细胞可以直接 从无血清或含血清培养基转入EX-CELL 420进行继代培养，无需改造。 EX-CELL 420中的培养物通常达到高于1 × 107 细胞/毫升的细胞密度，成活率大于95% 。悬浮培养物不用重新接种 即可维持生长10天以上。Sf9 和 Sf21细胞 在EX-CELL 420中可培养20多代也不出现 细胞活力损失。与含血清培养基相比，蛋白表达和病毒生产 得到改善。
EX-CELL 420 是一种完全培养基，针对 Sf9 和 Sf21 昆虫细胞系的无血清生长而开发和优化。细胞无需适应即可从无血清或加血清的培养基中直接转到 EX-CELL 420 中进行传代培养。EX-CELL 420 中的培养通常可达到高于 1×107 个/mL 的细胞密度，活性大于 95%。悬浮培养不添加培养基时可维持 10 天以上。Sf9 和 Sf21 细胞可在 EX-CELL 420 培养基中传代超过 20 次而不丧失活性。含血清培养基可提高蛋白质的表达和病毒的产量。
法律信息
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)
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