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U1250

Sigma-Aldrich

尿素

ReagentPlus®, ≥99.5%, pellets

别名:

碳酰二胺, 碳酰胺

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About This Item

线性分子式:
NH2CONH2
CAS号:
57-13-6
分子量:
60.06
Beilstein:
635724
EC 号:
200-315-5
MDL编号:
MFCD00008022
UNSPSC代码:
12352100
PubChem化学物质编号:
57654718
NACRES:
NA.21

质量水平

300

产品线

ReagentPlus®

方案

≥99.5%

表单

pellets

mp

132-135 °C (lit.)

溶解性

H2O: 8 M

密度

1.335 g/mL at 25 °C (lit.)

官能团

amine

SMILES字符串

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

基因信息

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

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相关类别

一般描述

尿素是离液剂，用于蛋白质变性。它扰乱了蛋白质的二级、三级和四级结构中的氢键。尿素还可以干扰 DNA 二级结构中存在的氢键。

应用

尿素已用作蛋白质变性剂。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。

法律信息

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000418590
0000418590
0000418589
0000418589
0000418591

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其他客户在看

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尿素 BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Sigma-Aldrich

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尿素 for biochemistry

Millipore

1.08488

尿素

尿素 crystalline, EMPROVE® ESSENTIAL, Ph. Eur., BP, ChP, JP, USP

SAFC

1.08486

尿素

Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Christos D Georgiou et al.
Redox biology, 17, 236-245 (2018-05-05)
A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and is based on their specific reaction with rhodamine B hydrazide (RBH), and the production of a protein carbonyl-RBH hydrazone the fluorescence of which (at ex/em
Christos D Georgiou et al.
Redox biology, 17, 128-142 (2018-04-24)
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
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实验方案

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

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