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Merck

B4380

Sigma-Aldrich

溴乙烷

≥97% purity, powder

别名:

单溴代二苯甲烷

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About This Item

经验公式(希尔记法):
C10H11BrN2O2
CAS号:
分子量:
271.11
Beilstein:
4430959
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

product name

溴乙烷, ≥97% (HPLC)

品質等級

化驗

≥97% (HPLC)

形狀

powder

顏色

yellow

mp

161 °C

溶解度

acetonitrile: 20 mg/mL

&epsilon ;(消光係數)

4.6-5.1 at 396-398 nm in H2O

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

−20°C

SMILES 字串

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI 密鑰

AHEWZZJEDQVLOP-UHFFFAOYSA-N

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一般說明

溴代双满又称单溴联苯胺。它还是一种已知的硫醇探针,是一种在光解反应后活化的荧光试剂。

應用

溴代双满用于高效液相色谱法硫醇测定,适于荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇。溴代双满已用作研究牛心线粒体寡霉素敏感性 ATP 酶的荧光标记。
此物质可用于体外游离硫化氢的定量测定。它已用于标记含有硫醇基的蛋白质。

生化/生理作用

溶液形式的溴代双满可与小分子硫醇基(如 GSH)和反应性蛋白硫醇基(如血红蛋白)反应。溴代双满与硫醇的反应为二级pH依赖性反应,且在与硫醇盐反应后,可活化水溶性荧光产物以便检测。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Differing effects of mechanical dough development and sheeting development methods on aggregated glutenin proteins.
Sutton KH, et al.
Cereal Chem., 80.6, 707-711 (2003)
N S Kosower et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(7), 3382-3386 (1979-07-01)
The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and dibromobimane are effective on intact cells while
Florencia Sardi et al.
Analytical biochemistry, 435(1), 74-82 (2013-01-09)
A method based on the differential reactivity of thiol and thiolate with monobromobimane (mBBr) has been developed to measure nucleophilicity and acidity of protein and low-molecular-weight thiols. Nucleophilicity of the thiolate is measured as the pH-independent second-order rate constant of
Joachim Volk et al.
Dental materials : official publication of the Academy of Dental Materials, 25(12), 1556-1563 (2009-09-01)
Camphorquinone (CQ) is cytotoxic in cell cultures. The mechanism of this toxic action, however, is not yet clearly understood. Aim of this investigation was to analyze the effects of non-irradiated CQ on intracellular formation of reactive oxygen species (ROS), intracellular
Michael Hall et al.
Proteomics, 10(5), 987-1001 (2010-01-06)
The light-dependent regulation of stromal enzymes by thioredoxin (Trx)-catalysed disulphide/dithiol exchange is known as a classical mechanism for control of chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but in all chloroplast

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