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Merck
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文件

94448

Sigma-Aldrich

革兰氏结晶紫 溶液

for microscopy

别名:

碱性紫3；龙胆紫；六甲基副玫瑰苯胺；甲基紫10B

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About This Item

分類程式碼代碼:
12171500
NACRES:
MA.02

等級

for microscopy

品質等級

200

產品線

BioChemika

形狀

liquid

儲存期限

limited shelf life, expiry date on the label

技術

microbe id | staining: suitable

顏色

purple to very dark purple

密度

0.981 g/mL at 20 °C

εmax

33.8 at 585-595 nm in water

適合性

suitable for microscopy

抗生素活性譜

Gram-negative bacteria
Gram-positive bacteria

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

相关类别

一般說明

革兰氏结晶紫溶液，也称为龙胆紫或六甲基副玫瑰苯胺，是一种基本、阳离子、嗜酸性氨基芳甲烷染料。它是六-N-甲基化的三氨基三苯甲烷，具有中等大小的亲脂性阳离子和轻微非平面的共轭体系。在细菌学中，它用作革兰氏染色的主要染料，以区分革兰氏阳性菌和革兰氏阴性菌。众所周知，它具有抗菌和抗真菌的特性。

應用

  • 结晶 紫常用于革兰氏染色及其变体、 染色淀粉样物质、细菌成分和维管植物组织。
  • 它用于环氧树脂切片的多色染色、 培养神经元的活性染色以及分析减数分裂结构的 共聚焦光学切片。
  • 它还被用于细胞内细菌、微孢子虫孢子和细胞学涂片的 吖啶橙-结晶紫染色。

生化／生理作用

该 结晶紫的抗菌作用来源于自由基的产生 和未电离染料-细菌复合物的形成。它还抑制 细菌蛋白质的合成和细胞壁的形成， 对革兰氏阳性菌尤其有效。

原則

结晶 紫是革兰氏染色的主要染色剂。它渗透到 革兰氏阳性细菌富含肽聚糖的细胞壁中， 并与革兰氏碘形成复合物，这些复合物被截留，即使在脱色后 也保持紫色。因此，革兰氏阳性细菌可以与 革兰氏阴性细菌区分开来，后者的肽聚糖层较薄， 不能保留结晶紫。

訊號詞

Warning

危險分類

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

93.2 °F

閃點（°C）

34 °C

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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分析证书(COA)

Lot/Batch Number
BCCL8948
BCCK2162
BCCL0880
BCCJ8029
BCCH7817

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