推荐产品
化驗
>98% (HPLC and SDS-PAGE)
形狀
suspension
包裝
pkg of 15 mL (05015979001 [settled resin volume])
pkg of 2 mL (11719408001 [settled resin volume])
pkg of 5 mL (11134515001 [settled resin volume])
製造商／商標名
Roche
容量
>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
儲存溫度
2-8°C
一般說明
重组蛋白A在蛋白A琼脂糖生产中的应用具有以下优点：它完全不含已知具有促有丝分裂作用的葡萄球菌肠毒素（分离自金黄色葡萄球菌的蛋白A中的污染物） 。固定化学可在广泛的pH和缓冲条件下提供高IgG的结合能力以及防漏稳定性。根据洗脱条件，凝胶可以使用30次以上。
蛋白A泄漏：<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98％纯度，HPLC，SDS-Page/1ml凝胶
蛋白A，固定化。
蛋白A泄漏：<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98％纯度，HPLC，SDS-Page/1ml凝胶
蛋白A，固定化。
特異性
蛋白A是从特定细菌菌株中分离的细胞壁蛋白，其具有来自不同物种的某些类免疫球蛋白的特异性结合位点。蛋白A可在不同程度结合IgM、IgA、IgD和大多数IgG亚类。
應用
从腹水或细胞培养上清液中纯化小鼠单克隆抗体。蛋白A或蛋白G琼脂糖亲和基质可用于从粗细胞提取物中纯化抗体 以及蛋白质的免疫沉淀或共免疫沉淀。它还被用于光活化-核糖核苷-增强的交联免疫沉淀（PAR-CLIP）分析。
成分
Preswollen凝胶，即用型，2ml或5ml（沉淀凝胶体积）悬浮于缓冲液中的凝胶，该缓冲液中含有20％乙醇作为保藏剂，非无菌。
蛋白A含量：3mg/ml预溶胀凝胶
蛋白A含量：3mg/ml预溶胀凝胶
品質
纯度：> 98％（HPLC，SDS-PAGE）；完全不含葡萄球菌肠毒素。
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
102.2 °F
閃點（°C）
39 °C
其他客户在看
Vladimir V Rogov et al.
EMBO reports, 18(8), 1382-1396 (2017-06-29)
Through the canonical LC3 interaction motif (LIR), [W/F/Y]-X1-X2-[I/L/V], protein complexes are recruited to autophagosomes to perform their functions as either autophagy adaptors or receptors. How these adaptors/receptors selectively interact with either LC3 or GABARAP families remains unclear. Herein, we determine
David Llobet-Navas et al.
Molecular and cellular biology, 34(23), 4216-4231 (2014-10-01)
Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1)
Florian Dunker et al.
Bio-protocol, 11(3), e3911-e3911 (2021-03-19)
Over the last decade, it has been noticed that microbial pathogens and pests deliver small RNA (sRNA) effectors into their host plants to manipulate plant physiology and immunity for infection, known as cross kingdom RNA interference. In this process, fungal
Xiuqing He et al.
Cells, 12(12) (2023-06-28)
The axoneme and accessory structures of flagella are critical for sperm motility and male fertilization. Sperm production needs precise and highly ordered gene expression to initiate and sustain the many cellular processes that result in mature spermatozoa. Here, we identified
R Travis Taylor et al.
Methods (San Diego, Calif.), 55(2), 166-171 (2011-08-23)
Ubiquitin (Ub) conjugation to a substrate protein is a widely used cellular mechanism for control of protein stability and function, modulation of signal transduction pathways and antiviral responses. Identification and characterization of ubiquitinated viral proteins is an important step in
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门