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10843555001

Roche

潮霉素B

from Streptomyces hygroscopicus

别名:

潮霉素B 来源于吸水链霉菌

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About This Item

经验公式（希尔记法）:
C20H37N3O13
CAS号:
31282-04-9
分子量:
527.52
MDL编号:
MFCD06795479
UNSPSC代码:
12352200
PubChem化学物质编号:
329749191

生物来源

Streptomyces hygroscopicus

无菌性

non-sterile; 0.2 μm filtered

方案

80% (HPLC)

表单

buffered aqueous solution

组成

Hygromycin B, >80% HPLC

包装

pkg of 20 mL (1 g)

制造商/商品名称

Roche

浓度

50 mg/mL

技术

transfection: suitable

SMILES字符串

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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相关类别

一般描述

  • 分子式：C20H37N3O13
  • LD50: 小鼠6 mg/kg（静脉注射）;豚鼠13 mg/kg（腹腔注射）; 大鼠63 mg/kg （腹腔注射）
  • 分子量：Mr = 527.5
  • 工作浓度：细胞培养中50 - 1,000 μg/ml。用于选择哺乳动物细胞的常用浓度是 200μ g/ml。。但是，最佳浓度必须通过实验确定，因为它可能会因使用的细胞类型而异。

应用

潮霉素 B 是一种氨基糖苷抗生素，可抑制原核生物和真核生物中的蛋白质合成。它被用于选择和维持可以稳定转染大肠杆菌潮霉素抗性基因（hyg 或 hph）的真核细胞的表型。

生化/生理作用

作用机制：本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。

抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作用。

质量

纯度：>80%（HPLC）

单位定义

单位试验：Unit/mg
我们没有这些信息。

外形

溶液，50 mg/ml，溶于PBS（磷酸盐缓冲生理盐水 ）中，已通过0.2 μm孔径滤膜过滤。

制备说明

工作浓度：在细胞培养中用于选择抗性细胞的推荐浓度是1000μ g/ml。 用于选择哺乳动物细胞的常用浓度是 200μ g/ml。 但是，最佳浓度必须通过实验确定，因为可能会因使用的细胞类型而异。

储存及稳定性

2至8 °C下储存。（溶液）

其他说明

仅用于生命科学研究。不用于诊断操作。
溶液，50 mg/ml，溶于PBS（磷酸盐缓冲生理盐水 ）中，已通过0.2 μm孔径滤膜过滤。

警示用语：

Danger

危险分类

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
71671300
61040600
57475600
46230600
37596700

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