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Merck
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WBKLS

Millipore

Immobilon Western 化学发光 HRP 底物

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

别名:

HRP substrate, chemiluminescent detection substrate, detection substrate

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About This Item

分類程式碼代碼:
41116010
eCl@ss:
42029053
NACRES:
NB.22

共軛

peroxidase conjugate

品質等級

200

包裝

pkg of 250 mL Luminol
pkg of 250 mL Peroxide solution

製造商／商標名

Immobilon®

技術

western blot: suitable

膜面積

5000 cm2 , membrane coverage

檢測方法

chemiluminescent

運輸包裝

ambient

相关类别

一般說明

Immobilon Western HRP底物与所有常用缓冲液和封膜试剂相容，在PVDF和硝化纤维膜上为蛋白质印迹或墨点/狭线/斑点印迹应用带来较高的化学发光检测灵敏度。

應用

Immobilon Western 化学发光HRP底物适用于蛋白质印迹。

法律資訊

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Nicolas Bidère et al.
Nature, 458(7234), 92-96 (2009-01-02)
The transcription factor NF-kappaB is required for lymphocyte activation and proliferation as well as the survival of certain lymphoma types. Antigen receptor stimulation assembles an NF-kappaB activating platform containing the scaffold protein CARMA1 (also called CARD11), the adaptor BCL10 and
Elizabeth I Vink et al.
Virology, 422(2), 317-325 (2011-11-30)
The adenovirus E4-ORF3 protein promotes viral replication by relocalizing cellular proteins into nuclear track structures, interfering with potential anti-viral activities. E4-ORF3 targets transcriptional intermediary factor 1 alpha (TIF1α), but not homologous TIF1β. Here, we introduce TIF1γ as a novel E4-ORF3-interacting
Reinhard David et al.
The European journal of neuroscience, 31(6), 978-993 (2010-04-10)
Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic transmission in ganglia of the autonomic nervous system. Here, we determined the subunit composition of hetero-pentameric nAChRs in the mouse superior cervical ganglion (SCG), the function of distinct receptors (obtained by deletions of
Novel irreversible epidermal growth factor receptor inhibitors by chemical modulation of the cysteine-trap portion.
Carmi, Caterina, et al.
Journal of medicinal chemistry, 53, 2038-2050 (2010)

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Dot/Slot (Filtration) Blotting

The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

实验方案

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This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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