推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
NEB-3F4, monoclonal
分子量
calculated mol wt 772.937 kDa
observed mol wt ~47-49 kDa
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100 μg
技術
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... NEB(4703)
一般說明
Nebulin (UniProt: P20929; also known as NEB, NEM2) is encoded by the NEB gene (Gene ID: 4703) in human. Nebulin is a highly conserved, muscle-specific protein that is located in the thin filament of striated muscle. It is involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. It is shown to bind and stabilize F-actin. Nebulin has a highly repetitive protein structure with about 97% of the protein consisting of modules of 30 to 35 amino-acids arranged into simple repeats or super repeats. Each repeat binds to actin along the length of the thin filament. The repeat modules contain conserved SDxxYK consensus sequence that binds F-actin. The N-terminal and the C-terminal ends of Nebulin contain unique protein domains. The C-terminal is anchored in the Z-disc of the muscle sarcomere and contains a conserved Src homology (SH3) domain. Although all known exons in the NEB gene can encode a protein with up to 8,525 amino-acids, alternative splicing may produce somewhat smaller proteins. Most of these alternative splicing events simply remove exons from the nebulin mRNAs, however, all nebulin mRNAs contain either exon 143 or exon 144, but never both. Exon 143 codes for amino acids 5242-5272 and exon 144 codes for amino acids 5277-5307. The sequence encoded by exon 143 shows complete homology between mouse, rat, and human. Studies on NEB knockout mice demonstrate a role for nebulin in establishing lateral connectivity of myofibrils and they lose their tight lateral register on stretch. (Ref.: Lam, L., et al. (2018). Sci. Rep. 8; 15728; Tonino, P., et al. (2010). J. Cell Sci. 123(3); 384-391; Donner, K., et al. (2004). Eur. J. Hum. Genet. 12, 744 751).
特異性
Clone NEB-3F4 is a mouse monoclonal antibody that detects Nebulin. It targets an epitope within the sequence encoded by exon 143 (aa 5242-5272).
免疫原
GST-tagged recombinant fragment corresponding to amino acids encoded by exons 138-143 expressed in E.coli.
應用
Quality Control Testing
Evaluated by Western Blotting in Nebulin recombinant fragments.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Nebulin fragments encoded by exons 138-143, but not exons 138-144.
Tested applications
Western Blotting Analysis: A representative lot detected Nebulin exon 143 in Western Blotting applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Immunofluorescence Analysis: A representative lot detected Nebulin exon 143 in Immunofluorescence applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Immunohistochemistry Applications: A representative lot detected Nebulin exon 143 in Immunohistochemistry applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Nebulin recombinant fragments.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Nebulin fragments encoded by exons 138-143, but not exons 138-144.
Tested applications
Western Blotting Analysis: A representative lot detected Nebulin exon 143 in Western Blotting applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Immunofluorescence Analysis: A representative lot detected Nebulin exon 143 in Immunofluorescence applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Immunohistochemistry Applications: A representative lot detected Nebulin exon 143 in Immunohistochemistry applications (Lam, L.T., et al. (2018). Sci Rep. 8(1):15728).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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