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生物源
mouse
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
6, monoclonal
物種活性
human
包裝
antibody small pack of 25 μg
技術
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
目標翻譯後修改
unmodified
基因資訊
human ... HSD3B2(3284)
一般說明
3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. (3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. 3-beta-HSD II is a single-pass membrane protein found on mitochondrial and endoplasmic reticulum membranes. It is expressed in adrenal gland, testis and ovary. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. Mutations in HSD3B2 gene are reported to cause adrenal hyperplasia that is characterized by androgen excess, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormone hypersecretion (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).
特異性
Clone 6 specifically detects HSDB32 in human tissue. it targets an epitope with in 14 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (3-beta-HSD).
應用
Anti-HSD3B2, clone 6, Cat. No. MABS2067, is a mouse monoclonal antibody that detects 3-beta-HSD II (HSD3B2) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B2 in Immunohistochemistry appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).
Western Blotting Analysis: Analysis: A representative lot detected HSD3B2 in Western Blotting appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).
Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected HSD3B2 in human adrenal gland and human placenta tissue sections.
ELISA Analysis: A representative lot detected HSD3B2 in ELISA appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).
Western Blotting Analysis: Analysis: A representative lot detected HSD3B2 in Western Blotting appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).
Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected HSD3B2 in human adrenal gland and human placenta tissue sections.
ELISA Analysis: A representative lot detected HSD3B2 in ELISA appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).
Research Category
Signaling
Signaling
品質
Evaluated by Western Blotting in human adrenal gland tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected HSD3B2 in human adrenal gland tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected HSD3B2 in human adrenal gland tissue lysate.
標靶描述
~40 kDa observed; 42.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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