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Merck
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MABC1108

Sigma-Aldrich

Anti-LAMP-1 Antibody, clone H4A3

clone H4A3, from mouse

别名:

Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.43

生物源

mouse

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

H4A3, monoclonal

物種活性

human

包裝

antibody small pack of 25 μg

技術

dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgGκ

NCBI登錄號

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

human ... LAMP1(3916)

一般說明

Lysosome-associated membrane glycoprotein 1 (UniProt: P11279; also known as LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a) is encoded by the LAMP1 gene (Gene ID: 3916) in human. LAMP-1 and LAMP-2 are two highly glycosylated proteins found in the lysosomal membrane. They may contribute up to 50% of all proteins present in the lysosomal membranes. They are known to maintain the lysosomal acidification and lysosomal membrane integrity. Extensive N-glycosylation on LAMP-1 and 2 to protects them from the lysosomal proteolysis. However, higher levels of poly-N-acetyllactosamines on LAMP-1 and 2 have been correlated with decreased cellular differentiation and enhanced metastatic potential. LAMP-1 is a single-pass type I transmembrane protein that contains a large lumenal domain (aa 29-382), a transmembrane domain (aa 383-405), and a short C-terminal cytoplasmic tail (aa 406-417). LAMP-1 is synthesized with a signal peptide (aa 1-28) that is subsequently cleaved in the mature form. LAMP-1 presents carbohydrate ligands to selectins and is also reported to act as a receptor for Lassa virus protein. Two isoforms of LAMP-1 have been described that are produced by alternative splicing. (Ref.: Tan, K-P., et al. (2016). Cell Death Dis. 7, e2347).

特異性

Clone H4A3 detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) in human cells.

免疫原

Human adherent peripheral blood cells.

應用

Anti-LAMP-1, clone H4A3, Cat. No. MABC1108, is a mouse monoclonal antibody that detects Lysosome-associated membrane glycoprotein 1 (LAMP-1) and has been tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected LAMP-1 in HeLa and A431 cells.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected LAMP-1 in Immunohistochemistry applications (Furuta, K., et. al. (2001). Am J Pathol. 159(2):449-55).

Western Blotting Analysis: A representative lot detected LAMP-1 in Western Blotting applications (Mane, S.M., et. al. (1989). Arch Biochem Biophys. 268(1):360-78).

Immunofluorescence Analysis: A representative lot detected LAMP-1 in Immunofluorescence applications (Starr, T., et. al. (2011). Cell Host Microbe. 11(1):33-45).

Dot Blot Analysis: A representative lot detected LAMP-1 in Dot Blot applications (Arruda, L.B., et. al. (2006). J Immunol. 177(4):2265-75).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected LAMP-1 in human kidney and human pancreas tissue sections.

Immunoprecipitation Analysis: A representative lot immunoprecipitated LAMP-1 in Immunoprecipitation applications (Mane, S.M., et. al. (1989). Arch Biochem Biophys. 268(1):360-78).
Research Category
Cell Structure

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LAMP-1 in HEK293 cell lysate.

標靶描述

~110 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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