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Merck
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MAB4360A4

Sigma-Aldrich

Anti-TRA-1-60 Antibody, clone TRA-1-60, Alexa Fluor 488 Conjugate

clone TRA - 1-60, from mouse, ALEXA FLUOR 488

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

ALEXA FLUOR 488

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

TRA - 1-60, monoclonal

物種活性

human

技術

immunocytochemistry: suitable

同型

IgM

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... PODXL(5420)

一般說明

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

特異性

This antibody reacts with TRA-1-60 antigen expressed upon the surface of human EC, EG, and ES cells. No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-81 (MAB4381A4) and TRA-1-60 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan.

免疫原

Human embryonal carcinoma cell line 2102Ep

應用

Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, Alexa Fluor 488 Conjugate validated for use in IC.
Research Category
Neuroscience

品質

Evaluated by Immunocytochemistry in H9 human embryonic stem cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.

外觀

size exclusion
Purified mouse monoclonal IgM conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
H9 human embryonic stem cells

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Conventional cell handling and sorting methods require manual labor, which decreases both cell quality and quantity. To purify adherent cultured cells, cell purification technologies that are high throughput without dissociation and can be utilized in an on-demand manner are expected.
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The network of stemness genes and oncogenes in human patient-specific reprogrammed cancer stem cells (CSCs) remains elusive, especially in liver cancer. HepG2-derived induced pluripotent stem cell-like cells (HepG2-iPS-like cells) were generated by introducing Yamanaka factors and the knockdown vector shTP53.
Ning Sun et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15720-15725 (2009-10-07)
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here
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Although several types of somatic cells have been reprogrammed into induced pluripotent stem cells (iPSCs) and then differentiated to hepatocyte-like cells (iHeps), the method for generating such cells from renal tubular epithelial cells shed in human urine and transplanting them
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Hepatocytes have been successfully generated from human pluripotent stem cells (hPSCs). However, the cost-effective and clinical-grade generation of hepatocytes from hPSCs still need to be improved. In this study, we reported the production of functional hepatocytes from clinical-grade human embryonic

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