MAB4145

Anti-BCRP Antibody, clone BXP-34

culture supernatant, clone BXP-34, Chemicon^®

• 别名: Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: BXP-34, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable
• 輸入: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• 同型: IgG1
• 適合性: not suitable for Western blot; not suitable for immunohistochemistry (Paraffin)
• NCBI登錄號: NM_004827.2
• UniProt登錄號: Q9UNQ0
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ABCG2(9429)

说明

一般說明

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.

特異性

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

免疫原

The Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.

應用

Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

Not suitable for paraffin-embedded tissues or for Western blotting

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

標靶描述

70 kDa

外觀

Liquid containing 0.7% BSA and 0.1% sodium azide.

儲存和穩定性

Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.

分析報告

Control
MCF-7 cells

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1