推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
QCRL-1, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
基因資訊
human ... ABCC1(4363)
特異性
Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
免疫原
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
應用
Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
外觀
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
儲存和穩定性
Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Cells resistant to HTI-286 do not overexpress P-glycoprotein but have reduced drug accumulation and a point mutation in alpha-tubulin.
Frank Loganzo, Malathi Hari, Tami Annable, Xingzhi Tan, Daniel B Morilla, Sylvia Musto et al.
Molecular Cancer Therapeutics null
S P Cole et al.
Science (New York, N.Y.), 258(5088), 1650-1654 (1992-12-04)
The doxorubicin-selected lung cancer cell line H69AR is resistant to many chemotherapeutic agents. However, like most tumor samples from individuals with this disease, it does not overexpress P-glycoprotein, a transmembrane transport protein that is dependent on adenosine triphosphate (ATP) and
M J Kelner et al.
Anti-cancer drugs, 11(3), 217-224 (2000-06-01)
This study is part of an effort to evaluate efficacy of the novel agent MGI 114 (HMAF) against tumors resistant to conventional chemotherapeutic agents. MGI 114 is a novel semisynthetic anticancer agent currently in chemotherapeutic phase II trials to evaluate
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British journal of pharmacology, 156(3), 497-508 (2009-01-22)
The function of transporters in peripheral blood mononuclear cells (PBMC) has been characterized, but less is known about cytochrome P450 (CYP) enzyme function in these cells. Given that cytokines are dysregulated in many diseases, the purpose of this work was
A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters.
J Chris Fromme,Jinoh Kim
Analytical biochemistry null
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