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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
KPL-1, monoclonal
物種活性
human
應無反應活性
rat (Snapp, KR et al 1998; Baisse, B et al 2007), pig (Snapp, KR et al 1998; Baisse, B et al 2007), bovine (Snapp, KR et al 1998; Baisse, B et al 2007), equine (Snapp, KR et al 1998; Baisse, B et al 2007)
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... SELPLG(6404)
一般說明
Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.
特異性
P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
免疫原
The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
應用
Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000
FACS {Snapp, KR et al 1998}.
Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).
Functional blocking of P-selectin {Snapp KR et al 1998}.
Immunohistochemistry: Fresh frozen, acetone fixed sections
Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200
Optimal working dilutions must be determined by the end user.
FACS {Snapp, KR et al 1998}.
Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).
Functional blocking of P-selectin {Snapp KR et al 1998}.
Immunohistochemistry: Fresh frozen, acetone fixed sections
Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200
Optimal working dilutions must be determined by the end user.
外觀
Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots up to 12 months.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Jessica M Banks et al.
PloS one, 8(11), e78988-e78988 (2013-11-19)
Stem bromelain, a cysteine protease isolated from pineapples, is a natural anti-inflammatory treatment, yet its mechanism of action remains unclear. Curious as to whether bromelain might affect selectin-mediated leukocyte rolling, we studied the ability of bromelain-treated human neutrophils to tether
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