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生物源
mouse
品質等級
共軛
ALEXA FLUOR™ 555
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
AP20, monoclonal
物種活性
rat, Xenopus, bovine, chicken, human, quail, mouse
技術
immunocytochemistry: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
chicken ... Map2(424001)
frog ... Map2(780043)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)
一般說明
微管相关蛋白2(MAP-2)是一种丰富的神经元细胞骨架蛋白,可与微管蛋白结合,稳定微管(Herzog and Weber, 1978),并且是神经元的极佳标记物。MAP-2对于神经元形态的发育和维持至关重要(Matus,1991)。已经在神经元中鉴定出MAP-2的三种主要亚型,即高分子量MAP2a和MAP2b,以及低分子量MAP2c(由MAP2基因的交替剪接产生)。(Chung et al.,1996)。低分子量亚型MAP2c在发育中的大脑中表达,并在大脑成熟过程中被下调,而高分子量MAP2b在发育中和成年大脑中均表达。MAP2a仅在大脑成熟后出现(Tucker,1990)。全部这些形式通过羧基末端附近的域与微管结合,该域包含31个氨基酸基序的三个或四个相似重复序列(Lewis et al.,1988)。MAP-2与MAP-4和tau蛋白一起属于与微管相关的热稳定蛋白家族。
應用
该抗MAP2抗体,克隆AP20,Alexa Fluor 555偶联物经验证可用于ICC中检测MAP2。
品質
通过免疫细胞化学在大鼠E18原代皮层细胞中进行评估。
免疫细胞化学分析:该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到MAP2。
免疫细胞化学分析:该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到MAP2。
標靶描述
观测值〜230 kDa。
外觀
在含 0.1% 叠氮化钠和 15 mg/mL BSA 的 PBS 中的Alexa Fluor™555偶联的纯化的小鼠单克隆 IgG。
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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