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Merck
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主要文件

MAB312

Sigma-Aldrich

抗A2B5抗体，克隆A2B5-105

clone A2B5-105, Chemicon®, from mouse

别名:

Neuron Cell Surface Antigen

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

100

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

A2B5-105, monoclonal

种属反应性（根据同源性预测）

mammals

制造商/商品名称

Chemicon®

技术

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

同位素/亚型

IgM

运输

wet ice

靶向翻译后修饰

unmodified

相关类别

特异性

与视网膜、脑、脊髓和背根神经节中神经元上的表面抗原反应，并与全部经检测的人神经母细胞瘤细胞系反应。似乎不与任何白血病细胞系或白血病患者的骨髓细胞结合，也不与正常骨髓细胞结合。在豚鼠补体存在下对神经元具有细胞毒性。蛋白A结合阳性。与迄今为止测试的全部物种的动物神经元质膜上的GQ神经节苷脂结合（Eisenbarth，et al.，1979）。

免疫原

鸡胚视网膜细胞

应用

使用经验证可用于FC、IC、IF、IH的抗A2B5抗体（克隆A2B5-105）检测A2B5。
研究子类别
神经 & 胶质标记
研究类别
神经科学
间接免疫荧光法（1：100-1：500）检测组织培养中的神经元。

使用双重免疫荧光，GQ神经节苷脂在神经元上具有与D2蛋白，破伤风毒素受体和神经丝相似的分布（Walsh，1980）。

也可用于消耗混合群组的神经元或通过亲和层析纯化它们。

也可用于检测神经母细胞瘤细胞向骨髓中的转移性扩散。

流式细胞术：活细胞{Maric, D. et al. (2000) Cerebral Cortex 10:729-747}。

免疫组化：冷冻、固定组织（Levison &McCarthy，1989）

补体介导的细胞毒性（Eisenbarth et al.，1979）

最佳工作稀释度必须由最终用户确定。

外形

形式：纯化
溶于0.02M PB，0.25M NaCl，pH 7.6，含有0.1％叠氮化钠的液体。

储存及稳定性

自发运之日起，在 2–8°C 条件下可稳定保存 1 年等分以避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。

分析说明

对照
II型星形胶质细胞，人类神经祖细胞

其他说明

浓度：请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Noah M Walton et al.
Development (Cambridge, England), 133(18), 3671-3681 (2006-08-18)
The isolation and expansion of human neural cell types has become increasingly relevant in restorative neurobiology. Although embryonic and fetal tissue are frequently envisaged as providing sufficiently primordial cells for such applications, the developmental plasticity of endogenous adult neural cells
Sebastián L Vega et al.
Experimental cell research, 351(1), 11-23 (2016-12-31)
Stem and progenitor cells that exhibit significant regenerative potential and critical roles in cancer initiation and progression remain difficult to characterize. Cell fates are determined by reciprocal signaling between the cell microenvironment and the nucleus; hence parameters derived from nuclear
Brittney A Beyer et al.
Nature chemical biology, 14(1), 22-28 (2017-11-14)
Endogenous metabolites play essential roles in the regulation of cellular identity and activity. Here we have investigated the process of oligodendrocyte precursor cell (OPC) differentiation, a process that becomes limiting during progressive stages of demyelinating diseases, including multiple sclerosis, using
Hala Gabr et al.
Cell transplantation, 24(9), 1813-1827 (2014-09-10)
Spinal cord injury (SCI) results in demyelination of surviving axons, loss of oligodendrocytes, and impairment of motor and sensory functions. We have developed a clinical strategy of cell therapy for SCI through the use of autologous bone marrow cells for
Zheng Chen et al.
Scientific reports, 9(1), 9437-9437 (2019-07-03)
Accumulation of iron has been associated with the pathobiology of various disorders of the central nervous system. Our previous work has shown that hephaestin (Heph) and ceruloplasmin (Cp) double knockout (KO) mice induced iron accumulation in multiple brain regions and
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