推荐产品
生物源
mouse
品質等級
共軛
ALEXA FLUOR™ 488
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
LM609, monoclonal
物種活性
rabbit, monkey, canine, avian, bovine, chicken, pig, human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunofluorescence: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ITGAV(3685)
相关类别
一般說明
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.
特異性
Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
免疫原
Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
應用
Flow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).Immunoprecipitation: Specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987).
Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.
Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL.
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.
Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor™488 conjugated is validated for use in FC, IF for the detection of Integrin αVβ3.
外觀
Protein A purified
Purified immunoglobulin from Protein A Sepharose™ chromatography, conjugated to Alexa Fluor 488. Final formulation in PBS 0.01M, SODIUM AZIDE 0.1%, PH7.1, 15mg/mL BSA.
儲存和穩定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).
NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV
Chen, 1995).
POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).
NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV
Chen, 1995).
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
免責聲明
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
C Andrew Boswell et al.
Molecular pharmaceutics, 5(4), 527-539 (2008-06-10)
Ligand size and valency strongly influence the receptor uptake and clearance of tumor angiogenesis imaging agents. The structures of successful imaging agents exhibit a high degree of variability, encompassing small monovalent arginine-glycine-aspartic acid (RGD)-containing peptides, multivalent RGD-oligomers, and a monoclonal
Andrew J Simnick et al.
ACS nano, 4(4), 2217-2227 (2010-03-26)
Multivalency is the increase in avidity resulting from the simultaneous interaction of multiple ligands with multiple receptors. This phenomenon, seen in antibody-antigen and virus-cell membrane interactions, is useful in designing bioinspired materials for targeted delivery of drugs or imaging agents.
Tanya Singh et al.
Journal of biomaterials applications, 29(3), 433-441 (2014-05-03)
The local microenvironment plays an important role in maintaining the dynamics of the extracellular matrix and the cell-extracellular matrix relationship. The extracellular matrix is a complex network of macromolecules with distinct mechanical and biochemical characteristics. Disruptions in extracellular matrix homeostasis
Mannose 6-phosphate/insulin-like growth factor 2 receptor limits cell invasion by controlling alphaVbeta3 integrin expression and proteolytic processing of urokinase-type plasminogen activator receptor.
Schiller, HB; Szekeres, A; Binder, BR; Stockinger, H; Leksa, V
Molecular Biology of the Cell null
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门