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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
AE5, monoclonal
物種活性
human, mouse, bovine, rabbit
製造商/商標名
Chemicon®
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG1
NCBI登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... KRT3(3850)
特異性
该抗体与角膜上皮的64 kDa多肽(细胞角蛋白3;角蛋白K3)和上颚上皮的角蛋白K76(细胞角蛋白K2p)反应。 因此,这代表了研究角膜上皮类型分化的极佳标记。也可识别牛口鼻和唇上皮以及兔角膜细胞系。
免疫原
兔角膜上皮角蛋白
應用
使用冷冻切片进行的免疫组化:1:50。
使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。
蛋白质印迹:1:500。
最佳工作稀释度必须由最终用户进行确定。
使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。
蛋白质印迹:1:500。
最佳工作稀释度必须由最终用户进行确定。
抗角蛋白K3/K76抗体,克隆AE5是抗角蛋白K3/K76的抗体,用于WB,IH,IH(P)。
標靶描述
64 kDa
外觀
形式:纯化
通过离子交换色谱法纯化单克隆抗体,将其置于含有0.09%叠氮化钠的磷酸盐缓冲生理盐水(pH 7.2)中。
儲存和穩定性
若购买后1个月内使用,则储存于2°-8°C,若要长期储存,则将抗体等分成小体积并储存于-20°C,保存达1年。
其他說明
浓度:请参考批次特异性浓缩物的检验报告。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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