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Key Documents

SCM012

Sigma-Aldrich

NDiff Neuro-2培养基补充剂 (200x)

NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation.

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.75

品質等級

形狀

liquid

製造商/商標名

Millipore Chemicon®

技術

cell culture | stem cell: suitable

輸入

sample type neural stem cell(s)

運輸包裝

dry ice

一般說明

Ndiff Neuro-2培养基补充剂最初由Austin Smith′s教授实验室开发,是一种无血清,类似N2的补充剂,用于将鼠胚胎干(ES)细胞体外分化为有丝分裂后神经元,尤其是通过单层分化。 该产品还可用于小鼠NS神经干细胞的衍生、繁殖和维持。

外觀

NDiff Neuro-2培养基补充剂包含胰岛素,脱铁转铁蛋白,亚硒酸钠,腐胺和孕酮。 该培养基补充剂以200X储备液的形式提供,应以1X的最终浓度添加到细胞培养基中。

儲存和穩定性

在-20°C下保存直至有效期。 避光保存。 解冻后,将未使用的部分等分至较小体积,并储存在-20°C下直至将来使用。 一旦添加到细胞培养基中,产品在4°C下可稳定保存四周。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Niche-independent symmetrical self-renewal of a mammalian tissue stem cell.
Conti, Luciano, et al.
PLoS Biology, 3, e283-e283 (2005)
Neural stem cell systems: diversities and properties after transplantation in animal models of diseases
Conti, Luciano, et al
Brain Pathology, 16, 143-154 (2006)
Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
Diogo, Maria Margarida, et al.
Biotechnology and Applied Biochemistry, 49, 105-112 (2008)
Adherent neural stem (NS) cells from fetal and adult forebrain
Pollard, Steven M, et al
Cerebral Cortex, 16 Suppl 1, i112-i120 (2006)
Dynamic cell-cell interactions between cord blood haematopoietic progenitors and the cellular niche are essential for the expansion of CD34+, CD34+CD38- and early lymphoid CD7+ cells.
da Silva, Claudia Lobato, et al.
Journal of Tissue Engineering and Regenerative Medicine, 4, 149-158 (2010)

商品

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

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实验方案

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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