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生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
mouse, monkey
物種活性(以同源性預測)
rat (based on 100% sequence homology)
包裝
antibody small pack of 25 μg
技術
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
目標翻譯後修改
phosphorylation (pSer586)
基因資訊
mouse ... Rasgrp2(19395)
一般說明
RAS guanyl-releasing protein 2 (UniProt: Q9QUG9; also known as Calcium and DAG-regulated guanine nucleotide exchange factor I, RasGRP2, CalDAG-GEFI, F25B3.3 kinase-like protein) is encoded by the Rasgrp2 gene (Gene ID: 19395) in murine species. RasGRP proteins are considered as activators of Ras and other related small GTPases by virtue o functioning as guanine nucleotide exchange factors (GEFs). RasGRP-1 through -4 share many structural domains, however, they display subtle differences between each of the different family members. They are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. RasGRP2′s expression is restricted to platelets and their precursors, megakaryocytes as well as neutrophils. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS, but not HRAS. It is also reported to functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils, probably through inside-out integrin activation. RaGRP2 is found both in the cytosol and associated with membranes. It is shown to be enriched at juxtamembrane areas and membrane ruffles. Three isoforms of RasGRP2 have been reported that are generated by alternative splicing. It contains two EF-hand domains, one N-terminal Ras-GEF domain, one phorbol-ester/DAG-type zinc finger domain, and one Ras-GEF domain. RasGRP2 deficient animals exhibit bleeding problems due to defects in platelets aggregation and degranulation. (Ref.: Olga Ksionda, O et al. (2013). Front Biol (Beijing). 8(5): 508 532).
特異性
This rabbit polyclonal antibody detects RAS guanyl-releasing protein 2 phosphorylated on Ser586 in Mouse and Monkey cells. It targets an epitope with in 11 amino acids surrounding Ser586 from the C-terminal region.
免疫原
A linear peptide corresponding to 11 amino acids surrounding phosphorylated Ser586 from the C-terminal region of murine RAS guanyl-releasing protein 2 .
Epitope: C-terminus
應用
Anti-phospho RasGRP2 (Ser586), Cat. No. ABS2118, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser586 and has been tested for use in Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser586) in Kinase assay with GST-Rasgrp2 (amino acids 1-374) and PKA, Forskolin and SKF81279 stimulated mouse striatal slices, COS-7 cells transfected with EGFP-Rasgrp2 and Myc-PKA, nucleus accumbens (NAc) from mice treated with cocaine, Nucleus accu (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).
Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser586) in RasGRP2 purified from GST-Rasgrp2 transfected COS-7 cells and phosphorylated by GST-PKAc alpha, Forskolin stimulated mouse striatal slices (Courtesy of Dr. Kozo Kaibuchi at Nagoya University, Japan).
Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser586) in RasGRP2 purified from GST-Rasgrp2 transfected COS-7 cells and phosphorylated by GST-PKAc alpha, Forskolin stimulated mouse striatal slices (Courtesy of Dr. Kozo Kaibuchi at Nagoya University, Japan).
品質
Evaluated by Western Blotting in RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser586 by GST-PKA catalytic subunit alpha (PKAc alpha)
Western Blotting Analysis): A 1:1,000 dilution of this antibody detected RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser586 by GST-PKA catalytic subunit alpha (PKAc alpha).
Western Blotting Analysis): A 1:1,000 dilution of this antibody detected RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser586 by GST-PKA catalytic subunit alpha (PKAc alpha).
標靶描述
~90 kDa observed; 69.45 kda calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 10% Ethylene Glycol, pH 7.6 with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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訊號詞
Warning
危險聲明
危險分類
STOT RE 2 Oral
標靶器官
Kidney
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
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