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Key Documents

AB5509

Sigma-Aldrich

抗阿片受体µ抗体

Chemicon®, from guinea pig

别名:

MOR-1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

guinea pig

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

primate, rat, mouse

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... OPRM1(4988)

特異性

Mu型阿片受体

免疫原

来自大鼠mu型阿片受体C末端的合成肽(目录号AG375),对应于大鼠mu阿片受体的残基384-398。

應用

免疫组化:1:50-500 参见方案。

免疫细胞化学:1:50-500 参见方案。

最佳工作稀释度必须由最终用户进行确定。

IHC方案: 雄性Sprague-Dawley大鼠(b.wt.100-150g)用戊巴比妥钠麻醉,并通过升主动脉灌注:1)50 mL不含Ca2+的台氏液,然后是2)多聚甲醛-苦味酸固定剂和3)10%蔗糖的PBS溶液作为冷冻保护剂。快速解剖组织,并在含有10%蔗糖的0.1 M磷酸盐缓冲液(pH值7.4)中保存过夜。将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。将一抗在封闭缓冲液中稀释至适当的工作稀释度。除去封闭缓冲液,然后将载玻片与AB5509在2-8°C下培养18-24小时。在PBS中冲洗3次后,将切片与Cy3偶联的二抗在室温下培养60分钟。固定在含有0.1%对苯二胺的PBS和甘油(1:3)的混合物中后,用Nikon Microphot-SA落射荧光显微镜检查切片。

ICC方案: 对Mu型阿片受体转染的细胞进行间接免疫荧光。除去培养基,并用无血清培养基轻轻洗涤细胞3次。除去培养基,并用无血清培养基轻轻洗涤细胞3次。固定后,如上所述对细胞进行间接免疫荧光处理。
抗阿片受体μ抗体-AB5509是用于IC,IH的针对阿片受体的抗体。
研究子类别
神经炎症&疼痛
研究类别
神经科学

聯結

替代品:AB1774

外觀

含50%甘油的PBS;浓度1.0 mg/ml

儲存和穩定性

自收到之日起,在-20° C以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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