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重組細胞
expressed in E. coli
化驗
≥90% (SDS-GE)
形狀
buffered aqueous solution
分子量
32,599.6 Da
溶解度
water: soluble
運輸包裝
ambient
儲存溫度
−20°C
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一般說明
Research area: Cell Struc
Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets: It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1). It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets: It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1). It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
應用
纳米盘可溶性脂质双层系统已被证明是一种广泛适用的手段，用于使膜蛋白溶在水溶液中的类似天然的双层膜环境中，维持其单分散和活性。纳米盘的关键组分是包绕着两亲性螺旋蛋白质带（膜支架蛋白）。
纳米盘系统已被用于包裹多种蛋白质，包括 GPCR、P450、细菌视紫红质、凝血因子、霍乱毒素、TAR 受体和芳香酶。
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
生化／生理作用
生成直径约 12.9nm 的纳米盘
法律資訊
纳米盘技术，以及它的许多用途，均由伊利诺伊大学所有的下列专利所保护。
- 7,691,414 膜支架蛋白
- 7,662,410 膜支架蛋白和嵌入膜蛋白
- 7,622,437组织因子组合物和方法
- 7,592,008 膜支架蛋白
- 7,575,763 膜支架蛋白和栓系膜蛋白
- 7,083,958 膜支架蛋白
- 7,048,949 膜支架蛋白
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Eliza A Ruben et al.
Blood, 139(24), 3463-3473 (2022-04-16)
The intrinsic and extrinsic pathways of the coagulation cascade converge to a common step where the prothrombinase complex, comprising the enzyme factor Xa (fXa), the cofactor fVa, Ca2+ and phospholipids, activates the zymogen prothrombin to the protease thrombin. The reaction
实验方案
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
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