推荐产品
产品名称
LY 294002, LY294002, CAS 154447-36-6, is a cell-permeable, potent, reversible, and specific inhibitor of PI 3-kinase ((IC₅₀ = 1.4 µM). Acts on the ATP-binding site.
质量水平
方案
≥98% (HPLC)
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
protect from light
颜色
off-white
溶解性
DMSO: 20 mg/mL
ethanol: soluble
运输
ambient
储存温度
−20°C
SMILES字符串
N4(CCOCC4)c1[o]c2c([c](c1)=O)cccc2c3ccccc3
InChI
1S/C19H17NO3/c21-17-13-18(20-9-11-22-12-10-20)23-19-15(7-4-8-16(17)19)14-5-2-1-3-6-14/h1-8,13H,9-12H2
InChI key
CZQHHVNHHHRRDU-UHFFFAOYSA-N
一般描述
一种可渗透细胞的，强效的，可逆的和特异性磷脂酰肌醇3-激酶（PI 3-激酶）抑制剂，可作用于酶的ATP结合位点（IC50 = 1.4 µ M）。还抑制460 kDa磷脂酰肌醇3样激酶DNA-PKcs中的非同源DNA末端连接（NHED），DNA-PKcs是DNA激活的蛋白激酶的催化亚基。在50 µM时不影响EGF受体激酶，MAP激酶，PKC，PI 4-激酶，S6激酶和c-src的活性。促进活性GSK-3b的核积累。据报告可抑制鼠胚胎干细胞的增殖。阻断培养的兔主动脉平滑肌细胞增殖，而不诱导凋亡。据报告可抑制RBL-2H3细胞中的组胺分泌（IC50 = 5 µM）。
一种细胞可渗透的、强效的、可逆的和特异性磷脂酰肌醇3-激酶抑制剂（IC50 = 1.4 µM），作用于酶的ATP结合位点。还抑制460 kDa磷脂酰肌醇3样激酶DNA-PKcs的非同源DNA末端连接（NHEJ）活性，DNA-PKcs是DNA激活的蛋白激酶的催化亚基。即使在50 µM时也不影响EGF受体激酶，MAP激酶，PKC，PI 4-激酶，S6激酶和c-Src的活性。促进活性GSK-3b的核积累。据报告可抑制鼠胚胎干细胞的增殖。阻断培养的兔主动脉平滑肌细胞增殖，而不诱导凋亡。显示与I-BET（目录号40101050 ~ 10 µM）结合在同一位点。也可购买10 mM（1 mg/325 µl）LY 294002（目录号440204） DMSO溶液。
生化/生理作用
主要靶标
磷脂酰肌醇3-激酶
磷脂酰肌醇3-激酶
产物与ATP竞争。
可逆性：是
细胞可渗透性：具有
靶标IC50：磷脂酰肌醇3-激酶为1.4 µM
警告
毒性：标准处理（A）
制备说明
实现完全溶解可能需要轻微加热。
重悬
复溶后，等分并冷冻保存（-20°C）。贮备液在-20°C下可稳定保存至多6个月。
其他说明
Dittman, A., et al. 2013. ACS Chem. Biol.9, 495.
Bechard, M., and Dalton.S. 2009.Mol.Cell.Biol.29, 2092.
Lianguzova, M.S. et al. 2007.Cell Biol. Int.31, 330.
Baumann, P., and West, S.C.1998.Proc.Natl.Acad.Sci. USA 95, 14066.
Cardone, M.H., et al. 1998.Science282, 1318.
Vlahos, C.J., et al. 1995.J. Immunol.154, 2413.
Yano, H., et al. 1995.Biochem.J.312, 145.
Vlahos, C.J., et al. 1994.J. Biol. Chem.269, 5241.
Selected Citations
Lee, J., et al. 2009.Cell Stem Cell5, 76.
Bechard, M., and Dalton.S. 2009.Mol.Cell.Biol.29, 2092.
Lianguzova, M.S. et al. 2007.Cell Biol. Int.31, 330.
Baumann, P., and West, S.C.1998.Proc.Natl.Acad.Sci. USA 95, 14066.
Cardone, M.H., et al. 1998.Science282, 1318.
Vlahos, C.J., et al. 1995.J. Immunol.154, 2413.
Yano, H., et al. 1995.Biochem.J.312, 145.
Vlahos, C.J., et al. 1994.J. Biol. Chem.269, 5241.
Selected Citations
Lee, J., et al. 2009.Cell Stem Cell5, 76.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
487.4 °F
闪点(°C)
253.0 °C
其他客户在看
Chengping Li et al.
International journal of oncology, 48(6), 2639-2646 (2016-04-02)
TRIP-Br1 oncoprotein is known to be involved in many vital cellular functions. In this study, we examined the role of TRIP-Br1 in hypoxia-induced cell death. Exposure to the overcrowded and CoCl2-induced hypoxic conditions increased TRIP-Br1 expression at the protein level
Shu Qi et al.
Cancer letters, 483, 98-113 (2020-03-29)
Liver metastases remain a major cause of death from gastrointestinal tract cancers and other malignancies, such as breast and lung carcinomas. Understanding the underlying biology is essential for the design of effective therapies. We previously identified the chemokine CCL7 and
Aadhya Tiwari et al.
Cancers, 12(10) (2020-10-03)
The multifunctional protein Y-box binding protein-1 (YB-1) regulates all the so far described cancer hallmarks including cell proliferation and survival. The MAPK/ERK and PI3K/Akt pathways are also the major pathways involved in cell growth, proliferation, and survival, and are the
Kelly H Forest et al.
Frontiers in molecular neuroscience, 14, 576038-576038 (2021-04-30)
Alzheimer's disease (AD) is the most common cause of dementia in the aging population. Evidence implicates elevated soluble oligomeric Aβ as one of the primary triggers during the prodromic phase leading to AD, effected largely via hyperphosphorylation of the microtubule-associated
Helga Simon-Molas et al.
International journal of molecular sciences, 22(14) (2021-07-25)
The glycolytic modulator TP53-Inducible Glycolysis and Apoptosis Regulator (TIGAR) is overexpressed in several types of cancer and has a role in metabolic rewiring during tumor development. However, little is known about the role of this enzyme in proliferative tissues under
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门