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重組細胞
expressed in E. coli
品質等級
形狀
liquid
比活性
≥10,000 units/mL
≥5,000 units/mg protein
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
技術
molecular cloning: suitable
異物活動
Nuclease-free
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
在大肠埃希菌中表达的重组HIV逆转录酶。催化DNA和RNA模板上容易出错的合成。为评估抗病毒药物提供了一个极好的靶标。
應用
大肠杆菌来源重组HIV 逆转录酶可用作逆转录酶活性测定标准品。
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
單位定義
一个单位定义为在37°C，pH 8.3下20分钟内将1.0 nmol标记的dTTP掺入酸不溶性物质中的酶量。
外觀
在10 mM磷酸钾，1 mM DTT，20%甘油（pH 7.4）中。
重構
初次融化后，等分并冷冻（-20°C）。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Geraldine Vilmen et al.
mBio, e0275221-e0275221 (2022-01-12)
Infection of rhesus macaques with simian-human immunodeficiency viruses (SHIVs) is the preferred model system for vaccine development because SHIVs encode human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Envs)-a key target of HIV-1 neutralizing antibodies. Since the goal of vaccines
Conformation of HIV-1 Envelope governs rhesus CD4 usage and simian-human immunodeficiency virus replication
Vilmen G, et al.
mBio, 13(1), e02752-e02721 (2022)
Identification of an antiretroviral small molecule that appears to be a host-targeting inhibitor of HIV-1 assembly
Reed JC, et al.
Journal of Virology, 95(3), 10-1128 (2021)
Bo Meng et al.
Cell reports, 35(13), 109292-109292 (2021-06-25)
We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer
Jonathan C Reed et al.
Journal of virology, 95(3) (2020-11-06)
Given the projected increase in multidrug-resistant HIV-1, there is an urgent need for development of antiretrovirals that act on virus life cycle stages not targeted by drugs currently in use. Host-targeting compounds are of particular interest because they can offer
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