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Merck
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Key Documents

17-500M

Millipore

Catch and Release v2.0 可逆免疫沉淀系统

别名:

Catch and release system, Immunoprecipitation kit

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NB.22

物種活性(以同源性預測)

all

品質等級

製造商/商標名

Catch And Release
Upstate®

技術

activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable

運輸包裝

wet ice

一般說明

特征&优点:
可重现:消除了通常导致减少/丢失样品的抽吸步骤
方便性:旋转柱形式极大地简化了样品处理
可逆:抗原:抗体复合物以天然或变性形式简单洗脱:



用途:
该试剂盒可通过使用旋转柱进行快速且可重现的免疫沉淀(IP)。 该系统比常规IP′具有更高的重现性,后者在不破坏琼脂糖床的情况下洗涤蛋白A/G琼脂糖方面存在问题。 抗体/抗原复合物在Catch and Release中的结合是可逆的,并且免疫复合物的洗脱可以用天然或变性缓冲液进行。 该系统已通过兔,小鼠,绵羊和山羊抗体成功测试。 使用人IgG1-4的IP应该是合适的。 本试剂盒不建议使用鸡抗体或人IgA、IgD、IgE或IgM进行IP。 使用前请阅读随附的产品手册。

應用

Catch and Release v2.0不应与含有6-His标签的蛋白质一起使用。

包裝

50次免疫沉淀

成分

Catch and Release 清洗缓冲液,10X(目录号20-210)

Catch and Release 非变性洗脱缓冲液,4X(目录号20-209)

Catch and Release 变性洗脱缓冲液,1X(目录号20-284)

20-285

20-216

Catch and Release 捕获管

品質

通过免疫沉淀进行了常规评估。

儲存和穩定性

全部组分应在4°C下储存,但Catch and Release捕获管除外,后者在室温下储存。 组件自装运之日起6个月内保持稳定。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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