1.09293

KOVACS′ indole reagent

for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology

• 别名: 4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles
• UNSPSC代码: 41106212
• NACRES: NA.21

属性

• 质量水平: 200
• 表单: liquid
• 包装: pkg of 100 mL
• 技术: microbe id | specific enzyme detection: suitable
• 颜色: yellow
• pH值（酸碱度）: <1 (20 °C in H₂O)
• 转变温度: flash point 36 °C
• 密度: 0.92 g/cm³ at 20 °C
• 应用: microbiology
• 储存温度: 2-8°C
• 适用性: Escherichia coli; enterovirulent E. coli (EEC) spp.

说明

一般描述

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

应用

Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

储存及稳定性

No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition

分析说明

Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).

其他说明

The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

安全信息

• 象形图: GHS02,GHS05,GHS07
• 警示用语：: Danger
• 危险声明: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 预防措施声明: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危险分类: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• 储存分类代码: 3 - Flammable liquids
• WGK: WGK 1
• 闪点(°F): 96.8 °F
• 闪点(°C): 36 °C