推荐产品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
技術
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pSer388)
基因資訊
human ... IRS2(8660)
一般說明
Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
特異性
This antibody recognizes IRS-2 phosphorylated at Ser388.
免疫原
Epitope: Phosphorylated Ser388
KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.
應用
Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.
Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
品質
Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
標靶描述
~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)
外觀
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
Untreated and insulin treated HEK293 cell lysates
Untreated and insulin treated HEK293 cell lysates
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Rania Alaaeldin et al.
Molecules (Basel, Switzerland), 26(24) (2021-12-25)
Insulin resistance contributes to several disorders including type 2 diabetes and cardiovascular diseases. Carpachromene is a natural active compound that inhibits α-glucosidase enzyme. The aim of the present study is to investigate the potential activity of carpachromene on glucose consumption
Dahae Lee et al.
Plants (Basel, Switzerland), 11(5) (2022-03-11)
Herein, we determined whether α-Spinasterol, a stigmastane-type phytosterol isolated from Aster pseudoglehnii, potentially impacts glucose uptake and glucose-stimulated insulin secretion in skeletal muscle cells and pancreatic β-cells, respectively. We observed that A. pseudoglehnii and its fractions enhanced glucose uptake, with
K I Rother et al.
The Journal of biological chemistry, 273(28), 17491-17497 (1998-07-04)
Insulin receptor substrates (IRSs) are tyrosine-phosphorylated following stimulation with insulin, insulin-like growth factors (IGFs), and interleukins. A key question is whether different IRSs play different roles to mediate insulin's metabolic and growth-promoting effects. In a novel system of insulin receptor-deficient
Reduced phosphorylation of brain insulin receptor substrate and Akt proteins in apolipoprotein-E4 targeted replacement mice.
Ong, QR; Chan, ES; Lim, ML; Cole, GM; Wong, BS
Scientific Reports null
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门