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Merck
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文件

05-591

Sigma-Aldrich

抗Akt / PKB抗体,PH结构域,克隆SKB1

clone SKB1, Upstate®, from mouse

别名:

Anti-AKT, Anti-PKB, Anti-PKB-ALPHA, Anti-PRKBA, Anti-RAC, Anti-RAC-ALPHA

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

SKB1, monoclonal

物種活性

rat, human, mouse

包裝

antibody small pack of 25 μg

製造商/商標名

Upstate®

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

mouse ... Akt1(11651)

一般說明

Akt(蛋白激酶B)是一种丝氨酸/苏氨酸激酶,是涉及细胞增殖、凋亡、血管生成和糖尿病的信号转导途径的关键酶。据报道,在哺乳动物中,Akt的三种同种型(α,β,γ 或Akt 1、2、3)显示高度同源性,但其调节性磷酸化位点略有不同。Akt α 是大多数组织中的主要同工型,Aktβ在胰岛素反应性组织中表达量最高,而Aktγ在脑组织中表达量丰富。每个Akt同种型由三个功能不同的区域组成:一个N末端pleckstrin homology(PH)结构域(它可提供一个脂质结合模块以将Akt引导至PIP2和PIP3上)、一个中央催化结构域和一个C末端疏水序列。

應用

使用此抗Akt/PKB抗体,PH结构域,克隆SKB1,检测Akt/PKB,经过验证可用于FC、IC、IP&WB。

品質

已通过免疫印迹法对A431细胞的RIPA裂解物进行了常规评估

標靶描述

60kda

外觀

免疫亲和力蛋白G纯化的免疫球蛋白,溶于0.02 M磷酸盐缓冲液(pH 7.6)、0.25M NaCl、0.1%叠氮化钠和30%甘油中。
形式:纯化

分析報告

对照
阳性抗原对照:货号12-301,未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液,煮沸5分钟以 减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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