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一般說明
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.
特異性
Recognizes Rho (-A, -B, -C), Mr 22 kDa.
免疫原
Recombinant human RhoA containing amino acids 1-155. Clone 55.
應用
Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
品質
Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.
標靶描述
22 kDa
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
分析報告
Control
HL-60 cell lysate.
HL-60 cell lysate.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Myrto Raftopoulou et al.
Developmental biology, 265(1), 23-32 (2003-12-31)
Rho GTPases control signal transduction pathways that link cell surface receptors to a variety of intracellular responses. They are best known as regulators of the actin cytoskeleton, but in addition they control cell polarity, gene expression, microtubule dynamics and vesicular
Regulation of cell function by Rho GTPases.
DerMardirossian, C and Bokoch, G M
Drug News & Perspectives, 14, 389-395 (2001)
Control of vesicular trafficking by Rho GTPases
Symons, Marc and Rusk, Nicole
Current Biology, 13, R409-R418 (2003)
Marina Sycheva et al.
Brain sciences, 9(8) (2019-08-23)
We have previously shown that the expression of pro-nerve growth factor (proNGF) was significantly increased, nerve growth factor (NGF) level was decreased, and the expression of p75NTR was enhanced in Alzheimer's disease (AD) hippocampal samples. NGF regulates cell survival and
Peroxynitrite mediates diabetes-induced endothelial dysfunction: possible role of Rho kinase activation.
El-Remessy, Azza B, et al.
Experimental Diabetes Research, 2010, 247861-247861 (2010)
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