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Merck

12072

Sigma-Aldrich

苯甲脒

≥95.0%

别名:

Benzenylamidine, Phenylamidine

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About This Item

线性分子式:
C6H5C(=NH)NH2
CAS号:
分子量:
120.15
Beilstein:
606020
EC 号:
MDL编号:
UNSPSC代码:
12352100
PubChem化学物质编号:
NACRES:
NA.22
价格与库存信息目前不能提供

质量水平

方案

≥95.0%

表单

powder or crystals

官能团

amine
phenyl

SMILES字符串

NC(=N)c1ccccc1

InChI

1S/C7H8N2/c8-7(9)6-4-2-1-3-5-6/h1-5H,(H3,8,9)

InChI key

PXXJHWLDUBFPOL-UHFFFAOYSA-N

应用

苯甲脒已用于从小鼠乳腺上皮细胞中制备富含硫酸乙酰肝素的蛋白多糖[1]。其已被用于防止牛因子XII(Hageman因子)纯化过程中的酶促降解[2]

生化/生理作用

苯甲脒是纤溶酶的抑制剂,已用于人血浆中胰高血糖素的放射免疫测定[3]。在促甲状腺激素释放激素(TRH)的放射免疫测定之前,其可防止体外添加至全血中的TRH的破坏[4]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Purification and characterization of bovine coagulation factor XII (Hageman factor).
H Claeys et al.
European journal of biochemistry, 87(1), 69-74 (1978-06-01)
Hung-Wei Chang et al.
PLoS pathogens, 15(6), e1007826-e1007826 (2019-06-21)
Vaccinia mature virus requires A26 envelope protein to mediate acid-dependent endocytosis into HeLa cells in which we hypothesized that A26 protein functions as an acid-sensitive membrane fusion suppressor. Here, we provide evidence showing that N-terminal domain (aa1-75) of A26 protein
Ana L Roa-Espitia et al.
Biology open, 5(9), 1189-1199 (2016-07-13)
Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function
Use of benzamidine as a proteolytic inhibitor in the radioimmunoassay of glucagon in plasma.
J W Ensinck et al.
The Journal of clinical endocrinology and metabolism, 35(3), 463-467 (1972-09-01)
M Jalkanen et al.
The Journal of cell biology, 101(3), 976-984 (1985-09-01)
Mouse mammary epithelial cells, of the normal murine mammary gland (NMuMG) cell line, bear a heparan sulfate-rich proteoglycan (HSPG) on their surfaces. A hybridoma (281-2) secreting a monoclonal antibody that recognizes this HSPG was produced by fusion of SP-2/0 myeloma

Questions

1–6 of 6 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. The buffer preparation protocol calls for benzamidine. Should I use the free base or the hydrochloride hydrate salt?

    1 answer
    1. It is possible that either the benzamidine or the benzamidine hydrochloride hydrate could work for your application. The major difference will be that the benzamidine hydrochloride hydrate (Product No. B6506) is expected to dissolve easier in aqueous solutions. Neither Product 12072 nor the salt Product B5606 is molecular biology, cell culture or biotechnology certified, so their performance once dissolved is likely to be similar.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What solvents can be used to dissolve Benzamidine, Product 12072?

    1 answer
    1. Based on our experience with this compound in quality control, we would expect it to be soluble in methanol (0.5g in 10mL of methanol).

      Helpful?

  6. What is the solution stability of Benzamidine, Product 12072?

    1 answer
    1. Sigma-Aldrich does not perform formal solution stability testing on this material; however, it is expected to be sensitive to oxidation. It is recommended to prepare solutions fresh for each use.

      Helpful?

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