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N3518

Sigma-Aldrich

NZ Amine® Broth

Lambda bacteriophage growth medium

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.85

grade

for molecular biology

Quality Level

100

sterility

non-sterile

form

powder

composition

MgSO4, 1 g/L
NaCl, 5 g/L
Tryptone, 10 g/L

pH

6.3-7.2(1.6% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

Suitable for non-selective cultivation of lambda bacteriophage.

Features and Benefits

NZ Amine powder provides:
  • Standard formulation
  • Convenient package sizes
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to liquid

Preparation Note

1. Suspend 15.94 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

N-Z-Amine is a registered trademark of Kerry Group

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yeast Extract for use in microbial growth medium

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Y1625

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Difco Manual, 356-356 (1998)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, A-A (1989)
F R Blattner et al.
Science (New York, N.Y.), 196(4286), 161-169 (1977-04-08)
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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