Skip to Content
Merck
All Photos(2)

Documents

L2897

Sigma-Aldrich

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Synonym(s):

Lennox broth, Luria Bertani Agar

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Looking for similar products? Visit Product Comparison Guide

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth with agar (Lennox) has been used for:

  • the transformation of competent E. coli cells
  • to cultivate Ecc-15 bacteria
  • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
  • transmission electron microscopy (TEM) and immunohistochemistry (IHC)
It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Easy scale-up using larger package sizes
  • Standard formulation

Preparation Note

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

related product

Product No.
Description
Pricing

L7275

LB Broth (Lennox), Tablet microbial growth medium

L7025

LB Broth with agar (Lennox), Tablet microbial growth medium

L7658

LB Broth (Lennox), EZMix powder microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix powder microbial growth medium

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Farbod Alimohammadi et al.
Langmuir : the ACS journal of surfaces and colloids, 34(24), 7192-7200 (2018-05-22)
Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive
Structural analysis of three-dimensional human neural tissue derived from induced pluripotent stem cells
Brooks PT, et al.
Journal of Separation Science, 6.0, 337-337 (2016)
Ultrastructural visualization of the Mesenchymal-to-Epithelial Transition during reprogramming of human fibroblasts to induced pluripotent stem cells
Hoffding, MK and Hyttel, P
Current Stem Cell Research & Therapy, 14.0, 39-53 (2015)
Patricia E Carreira et al.
Mobile DNA, 7, 21-21 (2016-11-16)
LINE-1 (L1) retrotransposons are a notable endogenous source of mutagenesis in mammals. Notably, cancer cells can support unusual L1 retrotransposition and L1-associated sequence rearrangement mechanisms following DNA damage. Recent reports suggest that L1 is mobile in epithelial tumours and neural
Martin Wegner et al.
Bio-protocol, 10(1), e3472-e3472 (2020-01-05)
Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA
View All Related Papers

Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service