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LVCAS9NEO

Sigma-Aldrich

Cas9 Neomycin Lenti Particles

Synonym(s):

Lenti Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

packaging

vial of 200 μL

Quality Level

200

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

application(s)

CRISPR

selection

neomycin / G418

shipped in

dry ice

storage temp.

−70°C

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Linkage

Click here to order gRNA plasmid and virus.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

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