MAB339
Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
clone BD24, Chemicon®, from mouse
Synonym(s):
Anti-DEE19, Anti-ECA4, Anti-EIEE19, Anti-EJM, Anti-EJM5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BD24, monoclonal
species reactivity
human, pig, avian, bovine
should not react with
feline, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GABRA1(2554)
Specificity
Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat
Immunogen
Epitope: N-terminus
Purified GABA/benzodiazepine receptor from bovine cortex.
Application
Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
Immunohistochemistry: 10-20 μg/mL * See protocol below.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Physical form
Format: Purified
Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Storage and Stability
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ivan Milenkovic et al.
Brain structure & function, 223(3), 1501-1518 (2017-11-24)
The function, regulation and cellular distribution of GABAA receptor subunits have been extensively documented in the adult rodent brain and are linked to numerous neurological disorders. However, there is a surprising lack of knowledge on the cellular (sub-) distribution of
Lik-Wei Wong et al.
Journal of neurochemistry, 134(5), 819-832 (2015-05-29)
GABAA receptors are pentameric ligand-gated ion channels that mediate inhibitory fast synaptic transmission in the central nervous system. Consistent with recent pentameric ligand-gated ion channels structures, sequence analysis predicts an α-helix near the N-terminus of each GABAA receptor subunit. Preceding
Raunak Sinha et al.
Cell, 168(3), 413-426 (2017-01-28)
The fovea is a specialized region of the retina that dominates the visual perception of primates by providing high chromatic and spatial acuity. While the foveal and peripheral retina share a similar core circuit architecture, they exhibit profound functional differences
Simone M Brändle et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(9) (2021-02-24)
Encephalitis associated with antibodies against the neuronal gamma-aminobutyric acid A receptor (GABAA-R) is a rare form of autoimmune encephalitis. The pathogenesis is still unknown but autoimmune mechanisms were surmised. Here we identified a strongly expanded B cell clone in the
Lindsey McCracken et al.
Neurology(R) neuroimmunology & neuroinflammation, 4(6), e404-e404 (2017-10-28)
We tested whether antibody screening samples of patients with suspected autoimmune encephalitis with additional research assays would improve the detection of autoimmune encephalitis compared with standard clinical testing alone. We examined 731 samples (333 CSF, 182 sera, and 108 pairs)
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