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432202

Sigma-Aldrich

Rhodamine 110 chloride

Dye content ≥75 %

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About This Item

Empirical Formula (Hill Notation):
C20H14N2O3 · HCl
CAS Number:
13558-31-1
Molecular Weight:
366.80
Beilstein:
4631860
EC Number:
236-944-7
MDL number:
MFCD00042009
UNSPSC Code:
12171500
PubChem Substance ID:
24867078
NACRES:
NA.47
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form

powder or crystals

Quality Level

200

composition

Dye content, ≥75%

mp

>300 °C (lit.)

λmax

496 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.Nc1ccc2c(OC3=CC(=N)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H

InChI key

JNGRENQDBKMCCR-UHFFFAOYSA-N

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General description

Rhodamine 110 chloride (R110) is a laser grade dye.[1]

Application

Rhodamine 110 chloride has been used for the synthesis of the rhodamine 110 octadecyl ester (R110C18).[2]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK2166
BCCH6230
BCCG1785
BCCD6833
SHBM0519

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Sean M Burrows et al.
The Analyst, 134(9), 1911-1921 (2009-08-18)
Steady-state single molecule fluorescence anisotropy (SMFA) is described to quantify free and bound probe molecules from a Biotin-Neutravidin complexation reaction. By formulating a ratio of bound to the total number of molecules sampled (N(b)/N(t) ratio) we quantified the extent of
Simeonika Rangełowa-Jankowska et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(13), 2449-2452 (2011-07-14)
The first observation of strong directional surface-plasmon-coupled emission (SPCE) of Rhodamine 110 in silica nanofilms deposited on silver nanolayers is reported. The preparation of the material is described in detail. The intensity of SPCE exceeds 10 times that of free
Ana Virel et al.
Analytical chemistry, 84(5), 2380-2387 (2012-02-14)
By site directed mutagenesis, we have produced recombinant mutants of human and mouse prethrombin-2 which are able to convert themselves autocatalytically into α-thrombin. We also have created a new method to amplify the signal of bioanalytical assays based on the
Thiemo Spielmann et al.
The journal of physical chemistry. B, 114(11), 4035-4046 (2010-03-04)
Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in a total internal reflection (TIR)
Soichiro Ijiri et al.
Electrophoresis, 32(24), 3499-3509 (2011-12-20)
We describe a highly sensitive CE with laser-induced fluorescence (LIF) detection for the analysis of N-linked oligosaccharides in glycoproteins using rhodamine 110 as a fluorescence derivatization reagent. One CE separation is performed using a fused-silica capillary and neutral pH buffer
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Questions

  1. What colour when soluble in water?

    1 answer

    1. Rhodamine 123 Choride is generally described as a green fluorescent dye. Excitation is around the 496-500 nm range. Excitation is around 520-522 nm. There is no specification for color once the dye is dissolved in a solvent.

      The colour of Rhodamine 110 Chloride in solid form is highly variable. The specification for 432202 states the color should be:
      Brown to Very Dark Brown and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown and Green to Very Dark Green.

      Rhodamine 110 chloride is only sparingly soluble in water. During quality control, product 432202 is dissolved in water at only 0.003 grams per liter. It is not advised to store Rhodamine 110 solutions dissolved in water. Solutions must be used fresh. Solubility is better in DMSO, DMF, Methanol and Ethanol.

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