This kit may be used to prepare gels at a range of 8 - 15%. An 8% gel requires 2.4 mL Resolving Solution, a 10% requires 3 mL, a 12% requires 3.6 mL, and a 15% requires 4.5 mL. See the table below for additional details. The feed ratio refers to the amount of acrylamide monomer to crosslinker.
Additional information may be accessed in the Product User Guide at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/613/20508849w-ms.pdf