STRN10

Spectrum^™ Plant Total RNA Kit

sufficient for 10 purifications

• Synonym(s): Plant Total RNA Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Properties

• usage: sufficient for 10 purifications
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): RNA purification: suitable
• test parameters: : ~30 min kit run time, sample volume: 100 mg
• greener alternative category: , Aligned
• storage temp.: 15-25°C

Description

General description

Click here to view the kit protocol

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Features and Benefits

• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Lysis Solution
• Binding Solution
• Wash Solution 1

Kit Components Also Available Separately

• M3148

  2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

  SDS

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• pcodes: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• target_organs: Liver,Heart
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• flash_point_f: 154.4 °F
• flash_point_c: 68 °C
• wgk: WGK 3