STRN50

Spectrum^™ Plant Total RNA Kit

sufficient for 50 purifications

• Synonym(s): Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Properties

• usage: sufficient for 50 purifications
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): RNA purification: suitable
• test parameters: : ~30 min kit run time, sample volume: 100 mg
• greener alternative category: Aligned,
• storage temp.: 15-25°C

Description

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Binding Solution
• Lysis Solution
• Wash Solution 1

Kit Components Also Available Separately

• M3148

  2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

  SDS

Safety Information

• wgk: WGK 3
• Storage Class: 10 - Combustible liquids