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Key Documents

ABC80

Sigma-Aldrich

Anti-phospho AMBRA1 Antibody (Ser52)

from rabbit, purified by affinity chromatography

Synonym(s):

Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer52)

Gene Information

human ... AMBRA1(55626)

General description

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells.

Specificity

This antibody recognizes AMBRA1 phosphorylated at Ser52.

Immunogen

Epitope: Phosphorylated Ser52
KLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52.

Application

Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA.

Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.

Target description

~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].).

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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