The recommended long-term storage temperature for the product is 2-8°C. The product is shipped on wet ice, indicating its sensitivity to temperature. However, there is no specific data available regarding temperature excursions or extended storage periods. It is the user's responsibility to assess whether the product is suitable for use if not stored according to the guidelines.
SRE0044
Trypsin inhibitor
lyophilized powder, suitable for mammalian cell culture
Synonym(s):
Trypsin inhibitor from Glycine max (soybean), SBTI
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Product Name
Trypsin Inhibitor from Glycine max (soybean), Suitable for manufacturing of diagnostic kits and reagents, suitable for cell culture
biological source
Glycine max (soybean)
Quality Level
form
lyophilized powder
mol wt
14 kDa
technique(s)
cell culture | mammalian: suitable
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL, clear, colorless to very faintly yellow
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
2-8°C
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Related Categories
Application
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Components
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Unit Definition
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What is the stability of the product SRE0044 if it is not stored at the recommended 2-8°C? Also, what form is the powder shipped in?
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