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C6164

Sigma-Aldrich

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture

Synonym(s):

cryopreservation medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

100

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).

Application

Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Bahari L, et al.
Testing, 13(2), e0192265-e0192265 (2018)
Antonia Patsialou et al.
Cancer research, 69(24), 9498-9506 (2009-11-26)
Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased
Zhenming Yu et al.
PloS one, 6(3), e17951-e17951 (2011-04-12)
Amyotrophic lateral sclerosis (ALS) is a devastating, rapidly progressive disease leading to paralysis and death. Recently, intermediate length polyglutamine (polyQ) repeats of 27-33 in ATAXIN-2 (ATXN2), encoding the ATXN2 protein, were found to increase risk for ALS. In ATXN2, polyQ
Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial
Lapa JA, et al.
Clinical and Vaccine Immunology : CVI (2008)
Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer
Plas E, et al.
Cytometry, 46(3), 184-189 (2001)
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Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Protocols

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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