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Key Documents

H4385

Sigma-Aldrich

Hanks′ Balanced Salt solution

10 ×, Modified, without calcium, magnesium or sodium bicarbonate

Synonym(s):

HBSS

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47

Quality Level

concentration

10 ×

pH

5.8-6.0

application(s)

hematology
histology

storage temp.

room temp

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General description

Hanks′ Balanced Salt solution (HBSS) is a cell and tissue culture solution. It is widely recommended for cell maintenance and conservation of tissues for a longer period. In addition, HBSS is used as a wet storage media for avulsed teeth, as it has the ideal osmolality and physiological pH for preserving the vitality of cells.

Application

Hanks′ Balanced Salt solution (HBSS) has been used for the elimination of the epithelial layer from the leukocytes and lymphocytes from the colon cells. It has also been used for the isolation of quail spermatozoa

Packaging

Contains: NaCl, 80 g/L; KCl, 4 g/L; glucose, 10 g/L; KH2PO4 600 mg/L; Na2HPO4 475 mg/L and phenol red, 170 mg/L

Other Notes

pH 6.9 when diluted according to instructions. Osmolality 240-250 mOsm/Kg.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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