Skip to Content
Merck
All Photos(1)

Key Documents

H1387

Sigma-Aldrich

Hanks′ Balanced Salts

Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

HBSS

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salts has been used:
  • for diluting digested tissues during isolation of pancreatic blood vessels
  • for washing mouse intestinal mucosal cells before lysis and sonication
  • as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone

Quantity

Formulated to contain 9.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Magalie Boucher et al.
Toxins, 13(3) (2021-03-07)
The fungal metabolite sporidesmin is responsible for severe necrotizing inflammation of biliary tract and liver of livestock grazing on pasture containing spores of Pithomyces chartarum that synthesizes the toxin. The toxin is secreted into bile causing the erosion of the
Till Jurgeleit et al.
Materials (Basel, Switzerland), 11(4) (2018-03-24)
FeMn alloys show a great potential for the use as a biodegradable material for medical vascular implants. To optimize the material properties, with respect to the intended application, new fabrication methods also have to be investigated. In this work different
Dajana Milovac et al.
Materials science & engineering. C, Materials for biological applications, 34, 437-445 (2013-11-26)
In the present study, poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone was prepared. Hydrothermal transformation of aragonitic cuttlefish bone into hydroxyapatite (HAp) was performed at 200°C retaining the cuttlebone architecture. The HAp scaffold was coated with a poly(ε-caprolactone) (PCL) using
Ehsan Mostaed et al.
Acta biomaterialia, 105, 319-335 (2020-01-27)
Zn-based alloys are recognized as promising bioabsorbable materials for cardiovascular stents, due to their biocompatibility and favorable degradability as compared to Mg. However, both low strength and intrinsic mechanical instability arising from a strong strain rate sensitivity and strain softening
Andrew I U Shearn et al.
Frontiers in cell and developmental biology, 8, 367-367 (2020-06-13)
Small extracellular vesicles (sEVs) are those nanovesicles 30-150 nm in size with a role in cell signalling and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) techniques are commonly used to measure sEV concentration in biofluids. However, this quantification

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service