It is recommended to reconstitute Chondroitinase ABC in a 0.1% BSA aqueous solution. Solubilizing 2 units in a 2 mL volume will result in a concentration of 2 units/mL or 0.002 units/µL.
C2905
Chondroitinase ABC from Proteus vulgaris
lyophilized powder, 0.3-3 units/mg solid
Synonym(s):
Chondroitin ABC Lyase
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About This Item
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Recommended Products
biological source
bacterial (Proteus vulgaris)
Quality Level
conjugate
(Glucosaminoglycan)
form
lyophilized powder
specific activity
0.3-3 units/mg solid
mol wt
~120 kDa by gel filtration
composition
Protein, ~35% biuret
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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Application
Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG).[1] The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems.[2] Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes.[3] The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.[4]
Biochem/physiol Actions
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.
Packaging
Packages based on chondroitinase C activity.
Unit Definition
One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts
Preparation Note
Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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Chondroitinase ABC from Proteus vulgaris C2905 2UNIT 용해 방법 문의 2unit Chondroitinase ABC을 0.002 unit/ul Chondroitinase ABC로 제조한다고 하면, 바로 완충용액 1ml를 넣어서 2unit/ml ->0.002unit/ul로 제조하는 것이 맞는지 확인요청드립니다.
1 answer-
Helpful?
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The specific activity range is 0.3-3unit/mg protein. I want to know something about it and how to use Product No. C2905.
1 answer-
The product datasheet contains additional information regarding this product, including usage instructions. Please see the link below to review this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/328/700/c2905dat-ms.pdf
To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific questions, so that a member of our team can reach out to you to assist further. Thank you.
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How should Product No. C2905 be reconstituted?
1 answer-
Reconstitute the product in a 0.01% bovine serum albumin aqueous solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% bovine serum albumin. Solutions should be prepared fresh.
Helpful?
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What is the composition of Product No. C2905?
1 answer-
This product consists of approximately 35% protein as determined by the Biuret method. BSA (bovine serum albumin) is a major component (~90%) of the protein. The remaining 65% is Tris buffer salts.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of this enzyme (Product No. C2905)?
1 answer-
Chondroitinase ABC is a lyase with a reported molecular weight of approximately 120 kDa by gel filtration and is composed of two subunits with molecular weights of 86 and 32.
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