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Key Documents

H8891

Sigma-Aldrich

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

Synonym(s):

Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Flavobacterium heparinum)

Quality Level

conjugate

conjugate (Glucosaminoglycan)

form

lyophilized powder

specific activity

≥30 units/mg protein (enzyme + BSA)

mol wt

70.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.

Biochem/physiol Actions

Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Unit Definition

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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