Skip to Content
Merck
All Photos(1)

Key Documents

MAB19562X

Sigma-Aldrich

Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated

clone D4B5, Chemicon®, from mouse

Synonym(s):

Anti-B2T, Anti-CSF, Anti-EBR2, Anti-EBR2A, Anti-LAMB2T, Anti-LAMNB2

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D4B5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAMC2(3918)

General description

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).

Specificity

Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 kDa (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.

Immunogen

Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein.
Epitope: amino acids 382-608

Application

Immunocytochemistry: 2 μg/mL
Flow cytometry
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated is validated for use in FC, IC for the detection of Laminin-5.

Physical form

Purified immunoglobulin conjugated to Alexa Fluor 488. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells.
Spassov, DS; Wong, CH; Wong, SY; Reiter, JF; Moasser, MM
Cancer Research null
Teresa Arnandis et al.
Developmental cell, 47(4), 409-424 (2018-11-21)
Centrosomal abnormalities, in particular centrosome amplification, are recurrent features of human tumors. Enforced centrosome amplification in vivo plays a role in tumor initiation and progression. However, centrosome amplification occurs only in a subset of cancer cells, and thus, partly due to
D J Devine et al.
Oncogene, 33(20), 2620-2628 (2013-06-19)
Epithelial-mesenchymal transition is one of the critical cellular programs that facilitate the progression of breast cancer to an invasive disease. We have observed that the expression of N-myc interactor (NMI) decreases significantly during progression of breast cancer, specifically in invasive

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service