GE28-9365-49

HiPrep^™ Heparin Fast Flow 16/10

Cytiva 28-9365-49

• Synonym(s): Heparin affinity chromatography
• UNSPSC Code: 13111000
• NACRES: NA.56

Properties

• ligand: porcine heparin
• form: prepacked column
• crosslinking: cross-linked
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9365-49
• storage condition: (20% ethanol containing 0.05 M sodium acetate)
• parameter: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 2 ml/min - 10 ml/min flow rate; 22 psi
• technique(s): liquid chromatography (LC): suitable
• bed size: 16 mm × 100 mm
• bed volume: 20 mL
• column I.D.: 16 mm
• matrix: 6% cross-linked agarose
• matrix active group: heparin
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 4-13
• working range: 4-12
• suitability: suitable for bioprocess medium
• separation technique: affinity

Description

General description

HiPrep^™ Heparin FF 16/10 is prepacked with Heparin Sepharose^™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Application

The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose^™ 6 Fast FLow with a chemically optimized linkage.

Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.

Features and Benefits

• 20 mL column optimized for convenient scale-up purification .
• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins.
• Simple operation with single pump configurations, as well as ÄKTA^® design systems.
• Excellent flow characteristics.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)

Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids