GE17-5247-01

HisTrap^™ High Performance

Cytiva 17-5247-01, pack of 5 × 1 mL

• Synonym(s): Nickel affinity chromatography
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• form: prepacked column
• crosslinking: cross-linked
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-5247-01
• storage condition: (20% Ethanol)
• parameter: 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature); 42 psi (H₂O at room temperature.)
• technique(s): liquid chromatography (LC): suitable
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked 6% agarose
• matrix active group: Nickel
• avg. part. size: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacity: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(H20 at room temperature); ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
• separation technique: affinity

Description

General description

HisTrap^™ HP columns are prepacked with Ni Sepharose^™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

HisTrap^™ HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose^™ High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

HisTrap^™ HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.

The high stability and broad compatibility of Ni Sepharose^™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep^™ FF 16/10 columns. Ni Sepharose^™ 6 Fast Flow, the medium prepacked in HisTrap^™ FF and HisPrep^™ FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.

Features and Benefits

• High binding capacity, at least 40 mg/mL chromatography medium.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible Ni²⁺ leakage
• Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisPrep is a trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS09
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids