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T3285

Sigma-Aldrich

TNM-FH insect medium

With ʟ-glutamine and sodium bicarbonate,liquid, sterile-filtered, suitable for insect cell culture

Synonyme(s) :

supplemented Grace′s medium

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About This Item

Numéro MDL:
MFCD00243216
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

TNM-FH insect medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Niveau de qualité

300

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | insect: suitable

Composants

NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Several modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species. 

Application

TNM-FH insect medium has been used to culture Sf9 insect cells.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Thomas Piechulek et al.
The Journal of biological chemistry, 280(47), 38923-38931 (2005-09-21)
The regulation of the two isoforms of phospholipase C-gamma, PLCgamma(1) and PLCgamma(2), by cell surface receptors involves protein tyrosine phosphorylation as well as interaction with adapter proteins and phosphatidylinositol 3,4,5-trisphosphate (PtdInsP(3)) generated by inositol phospholipid 3-kinases (PI3Ks). All three processes
J J Gallardo-Rodríguez et al.
Bioresource technology, 200, 699-705 (2015-11-12)
A modification of a flow contraction device was used to subject shear-sensitive microalgae to well-defined hydrodynamic forces. The aim of the study was to elucidate if the inhibition of shear-induced growth commonly observed in dinoflagellate microalgae is in effect due
Effects of media constituents on growth of insect cells in stationary and suspension cultures.
Hink, W.F., et al.
In Vitro, 9, 371-371 (1974)
Boglárka Zámbó et al.
Scientific reports, 8(1), 7487-7487 (2018-05-12)
The ABCG2 membrane protein is a key xeno- and endobiotic transporter, modulating the absorption and metabolism of pharmacological agents and causing multidrug resistance in cancer. ABCG2 is also involved in uric acid elimination and its impaired function is causative in
Established insect cell line from the cabbage looper, Trichoplusia ni.
W F Hink
Nature, 226(5244), 466-467 (1970-05-02)
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